Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 24)

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Start Up System (Bio-Plex 100, 200, or similar)
1. Empty the waste bottle and fill the sheath fluid bottle before starting if
high throughput fluidics (HTF) are not present. This will prevent fluidic
system backup and potential data loss.
2. Turn on the reader, XY platform, and HTF (if included). Allow the
system to warm up for 30 min (if not already done).
3. Select Start up and follow the instructions. If the system is idle
for 4 hr without acquiring data, the lasers will automatically turn off.
To reset the 4-hr countdown, select Warm up
and wait for the
lasers/optics to reach operational temperature.
Calibrate System
4. Select Calibrate and confirm that the default values for CAL1
and CAL2 are the same as the values printed on the bottle of Bio-Plex
calibration beads. Use the Bio-Plex system low RP1 target value
even if the assays will be run at high RP1. Bio-Plex Manager version
6.1 and higher will automatically calibrate at both high and low RP1
settings although only the low RP1 value option is listed under CAL2.
5. Select OK and follow the software prompts for step-by-step
instructions for CAL1 and CAL2 calibration.
Note : In Bio-Plex Manager version 6.1 and higher, startup, warm up,
and calibration can be performed together by selecting the Start up and
calibrate icon.
Prepare Protocol in Bio-Plex Manager Software
Version 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes in the assay, the plate wells to be
read, sample information, and instrument settings.
Bio-Plex Manager software versions 6.0 and higher contain protocols for
most Bio-Plex assays. Choose from available protocols or create a new