Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 28)

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Acquire Data
1. Shake the assay plate at 900–1,100 rpm for 30 sec, and visually inspect
the plate to ensure that the assay wells are filled with buffer. Slowly
remove the sealing tape before placing the plate on the plate carrier.
2. Click Run Protocol, and on the pop-up screen, select Load Plate
and click OK to start acquiring data.
3. Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
4. If acquiring data from more than one plate, empty the waste bottle
and refill the sheath bottle after each plate (if HTF is not present).
Select Wash Between Plates and follow the instructions. Then
repeat the Prepare Protocol and Acquire Data instructions.
5. When data acquisition is complete, select Shut Down and
follow the instructions.
Reacquire Data
It is possible to acquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in the Run Protocol step.
Any previous data will be overwritten unless the second run is saved under
a different file name.
1. Check the wells from which data will be reacquired.
2. Aspirate the buffer with the wash method of choice, but do not
perform wash step.
3. Add 100 µl of resuspension buffer to each well. Cover the plate with a
new sheet of sealing tape and shake plate at 900–1,100 rpm for 30 sec.
4. Repeat the Acquire Data steps to reacquire data. The data acquired
should be similar to those acquired initially; however, the acquisition
time will be extended because the wells have fewer beads.