Bio-Rad DCode™ Universal Mutation Detection System User Manual
Page 20
Fig. 7.3. Printed graph and sequence information.
17
DNA melting profile graph
Betaglobin
Sequence position (bp)
Betaglobin exon 1 with GC-clamp
Melting probability: 50%
GGGCTGGGCA
TAAAAGTCAG
GGCAGAGCCA
TCTATTGCTT
ACATTTGCTT
50
CTGACACAAC
TGTGTTCACT
AGCAACCTCA
AACAGACACC
ATGGTGCACC
100
TGACTCCTGA
GGAGAAGTCT
GCCGTTACTG
CCCTGTGGGG
CAAGGTGAAC
150
GTGGATGAAG
TTGGTGGTGA
GGCCCTGGGC
AGGTTGGTAT
CAAGGTTACA
200
AGACAGGTTT
AAGGAGACCA
ATAGAAACTG
GGCATGTGGA
GACAGAGAAG
250
ACTCTTGGGT
TTCTGATAGG
CACTGACTCT
CTCTGCCTAT
TGGTCTATTC
300
GGGCGGGGGC
GGCGGGACGG
GCGCGGGGCG
CGGCGGGCG
399
Mutant Betaglobin exon 1 with GC-clamp
Melting probability: 50%
GGGCTGGGCA
TAAAAGTCAG
GGCAGAGCCA
TCTATTGCTT
ACATTTGCTT
50
CGGACACAAC
TGTGTTCACT
AGCAACCTCA
AACAGACACC
ATGGTGCACC
100
TGACTCCTGA
GGAGAAGTCT
GCCGTTACTG
CCCTGTGGGG
CAAGGTGAAC
150
GTGGATGAAG
TTGGTGGTGA
GGCCCTGGGC
AGGTTGGTAT
CAAGGTTACA
200
AGACAGGTTT
AAGGAGACCA
ATAGAAACTG
GGCATGTGGA
GACAGAGAAG
250
ACTCTTGGGT
TTCTGATAGG
CACTGACTCT
CTCTGCCTAT
TGGTCTATTC
300
GGGCGGGGGC
GGCGGGACGG
GCGCGGGGCG
CGGCGGGCG
399
100.0
94.0
88.0
82.0
76.0
70.0
1
69
137
205
273
341
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