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Bio-Rad DCode™ Universal Mutation Detection System User Manual

Page 20

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Fig. 7.3. Printed graph and sequence information.

17

DNA melting profile graph

Betaglobin

Sequence position (bp)

Betaglobin exon 1 with GC-clamp
Melting probability: 50%
GGGCTGGGCA

TAAAAGTCAG

GGCAGAGCCA

TCTATTGCTT

ACATTTGCTT

50

CTGACACAAC

TGTGTTCACT

AGCAACCTCA

AACAGACACC

ATGGTGCACC

100

TGACTCCTGA

GGAGAAGTCT

GCCGTTACTG

CCCTGTGGGG

CAAGGTGAAC

150

GTGGATGAAG

TTGGTGGTGA

GGCCCTGGGC

AGGTTGGTAT

CAAGGTTACA

200

AGACAGGTTT

AAGGAGACCA

ATAGAAACTG

GGCATGTGGA

GACAGAGAAG

250

ACTCTTGGGT

TTCTGATAGG

CACTGACTCT

CTCTGCCTAT

TGGTCTATTC

300

GGGCGGGGGC

GGCGGGACGG

GCGCGGGGCG

CGGCGGGCG

399

Mutant Betaglobin exon 1 with GC-clamp
Melting probability: 50%
GGGCTGGGCA

TAAAAGTCAG

GGCAGAGCCA

TCTATTGCTT

ACATTTGCTT

50

CGGACACAAC

TGTGTTCACT

AGCAACCTCA

AACAGACACC

ATGGTGCACC

100

TGACTCCTGA

GGAGAAGTCT

GCCGTTACTG

CCCTGTGGGG

CAAGGTGAAC

150

GTGGATGAAG

TTGGTGGTGA

GGCCCTGGGC

AGGTTGGTAT

CAAGGTTACA

200

AGACAGGTTT

AAGGAGACCA

ATAGAAACTG

GGCATGTGGA

GACAGAGAAG

250

ACTCTTGGGT

TTCTGATAGG

CACTGACTCT

CTCTGCCTAT

TGGTCTATTC

300

GGGCGGGGGC

GGCGGGACGG

GCGCGGGGCG

CGGCGGGCG

399

100.0

94.0

88.0

82.0

76.0

70.0

1

69

137

205

273

341