Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 17)

When a project is saved, all sequences, sequence descriptions, and options are stored in
the project file. The actual melting profiles are not stored in the project file. The melting
profiles and graphs are calculated when the file is open. The calculation also takes place
automatically when a sequence is imported, when the sequence is edited, and when the
melting probability setting is changed.
Section 6
The Sequence Display
The Sequence Display is a window that shows information on a sequence from the
Project View (Figure 6.1).
Fig. 6.1. The Sequence Display window.
When a sequence is present in the Sequence Display window it can be viewed and edited.
A new sequence can be typed into the window using the keyboard. The window will show up
to 500 nucleotides divided on 10 lines of 50 nucleotides each. Use the scroll bar to see a
different part of the sequence. A sequence up to 10,000 nucleotides can be entered (9,000 in
Windows 3.1x). Characters different from "acgtu and ACGTU" will not be accepted. Both
upper and lower case letters can be used.
When editing a sequence the Cut, Copy, Paste, and Select All functions are active. For
WinMelt users these functions can be found under the Edit menu in the Sequence Display
window and these functions can also be activated by clicking the right button on the mouse.
For MacMelt users these functions can be found under the Edit menu in the Menu heading
at the top of the screen.
The melting probability calculation can be set to 5%, 25%, 50%, 75% or 95% by using
the arrow scroll button. The default melting probability setting is 50%. The melting profile data
for the sequence can be graphed when the Show graph box is checked. Uncheck the Show
graph box if the sequence is not to be graphed. The sequence name can be changed by
typing in a new name in the sequence description box.
If the sequence or the melting probability settings have changed, the melting profile will
be recalculated and the Graph View will be updated. Click OK to exit the Sequence Display
or click Cancel to close the Sequence Display without saving changes to the sequence or the
other options.
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