Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 10)

should only take a fraction of a second. However, if the sequence is long or if the computer
is not fast, a delay may be noticed.
Fig. 3.6. Project View window with wild-type and mutant TP53ex8 sequences.
3.5 Printing a Sequence
To print the mutant sequence, first select the "TP53ex8 mutant" sequence in the Project
View by clicking on the sequence icon, then select Print from the File menu. The standard
Print dialog box will appear. If necessary, select any print options and then click OK. To see
what will be printed before printing, use the Print Preview command on the File menu. To
print both the wild-type and mutant sequences, click on the mutant sequence icon so that
neither sequences are selected. Then print the sequences by selecting the Print command.
3.6 The Melting Profile Graph
To see the melting profile for the two sequences, select Show from the Graph menu.
The Graph View window will appear as shown in Figure 3.7. The abscissa (x-axis)
corresponds to the sequence position and the ordinate (y-axis) corresponds to the melting
midpoint in degrees Celsius (°C). The wild-type sequence is plotted in black, the mutant is plot-
ted in yellow.
Fig. 3.7. Graph View window with wild-type and mutant TP53ex8 sequences.
3.7 Editing the Graph axis
The axis of the graph can be changed by selecting Edit Graph Axis from the Graph
menu. Change the temperature range to 60 °C for the low and 100 °C for the high and then
click OK (Figure 3.8). The melt profile data will be graphed using the new coordinates. The
axis can also be changed by selecting an area inside the graph. Using the left mouse button,
click and drag an area in the graph. The melt profile will be graphed to the new coordinates.
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