Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 18)

Section 7
The Graph View
The Graph View shows the melting profile graphs of the sequences in the project that
were selected to be graphed (Figure 7.1). A legend with the colors for the different sequences
and a description of each graphed sequence is shown. Up to ten graphs can be shown
simultaneously in the Graph View.
Fig. 7.1. The Graph View window.
The title of the window contains the file name of the project. The melting probability
graphed will be the one selected for each sequence. The melting profile graphs of the sequences
are shown in different colors (black, red, green, blue).
The Graph View and related information are manipulated using the commands on the
Graph menu. The graph menu commands are:
Show or Hide Graph Displays or hides the Graph View window.
Edit Graph Axis Displays a window for changing the sequence range and temper-
ature range coordinates.
Default Graph Axis The temperature range will be set to 20–120 °C and the sequence
range will include the longest sequence in the project.
Export Graph Exports the melting profile graph as a image file.
The Graph View can be stretched to any size. The graph will automatically reformat to
fit the size of the window. The Show or Hide Graph command on the Graph menu or the
Show or Hide Graph button in the toolbar is used to show or hide this view.
There are two methods for adjusting the graph axis. One method is to use the mouse to
select the part of the graph you are interested in. Click and drag the area to be graphed using
the left mouse button. The axis will change to fit the selected area. The other method for
changing the axis is by using the Edit Graph Axis command on the Graph menu or the Edit
Graph Axis button in the toolbar (Figure 7.2). The sequence and temperature range can be
changed by typing in the new values. The graph can be zoomed out to a full view by the
Default Graph Axis command on the Graph menu. WinMelt users can also use the right
mouse button.
15