Bio-Rad Helios® Gene Gun System - Manual (Page 51)

10.9 Quick Guide to Operation
Before the Bombardment
1. Coat microcarriers with DNA, load into tubes, and prepare cartridges prior to day of exper-
iment.
2. Check helium supply (50 psi in excess of desired rupture pressure).
3. Clean and/or sterilize the Gene Gun, tube holders, and barrel liner as appropriate.
4. Connect the Gene Gun to a helium source.
5. Activate the Gene Gun: turn on the flow of helium to the desired pressure and with an empty
cartridge holder in place, make 2–3 “pre-shots” by engaging the safety interlock and firing
the trigger.
Firing the Device
1. Load cartridges into the cartridge holder and place in Gene Gun.
2. Prepare/position target cells for bombardment.
3. Bombard sample: engage safety interlock and press the firing trigger
After the Bombardment
1. Remove cartridge holder from Gene Gun.
2. Remove cartridges from cartridge holder.
3. Turn off the helium pressure to the system.
4. Turn the regulator value counterclockwise to de-pressurize the system.
5. Disconnect the helium hose and Gene Gun.
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