Bio-Rad Helios® Gene Gun System - Manual (Page 4)

Table of Contents
Page
Section 1 General Safety Information....................................................................1
1.1 Helios Gene Gun Safety........................................................................................1
1.2 Pressurized Helium and Nitrogen Safety..............................................................1
1.3 Power Safety..........................................................................................................1
1.4 Ear and Eye Protection..........................................................................................2
Section 2 Introduction to Particle Delivery ...........................................................2
2.1 Particle Delivery Technology ...............................................................................2
2.2 The Helios Gene Gun............................................................................................3
2.3 Operating Principle of the Helios Gene Gun System...........................................3
2.4 Requirements for System Operation.....................................................................4
Section 3 Product Description ................................................................................7
3.1 Packing List ...........................................................................................................7
3.2 Identification of System Components and Controls.............................................8
Section 4 Setting up the Helios Gene Gun System .............................................12
4.1 Inserting the Battery into the Helios Gene Gun .................................................12
4.2 Connecting the Helios Gene Gun to a Helium Source.......................................13
4.3 Connecting the Tubing Prep Unit to a Nitrogen Source ....................................14
Section 5 Operation of the Helios Gene Gun System.........................................17
5.1 Quick Guide to Operation ...................................................................................17
5.2 Preparation of System Components Prior to Bombardment ..............................18
5.3 Particle delivery using the Helios Gene Gun......................................................23
5.4 Removing Used Cartridges, Depressurization, and Shut Down ........................28
Section 6 Preparation of Mammalian Cell Targets............................................29
6.1 In vitro Delivery to Adherent Cells ....................................................................29
6.2 In vitro Delivery to Suspension Cultures............................................................30
6.3 in vivo Delivery to Epidermis .............................................................................31
Section 7 Optimization of Gene Gun Parameters ..............................................32
7.1 Overview .............................................................................................................32
7.2 Parameters
for in vitro Delivery..........................................................................34
7.3 Parameters for in vivo Delivery ..........................................................................35
Section 8 Troubleshooting .....................................................................................35
8.1 DNA/Microcarrier Preparation ...........................................................................35
8.2 Cartridge Preparation ..........................................................................................35
8.3 Helios Gene Gun Operation................................................................................36
8.4 In Vitro and in Vivo Targeting ............................................................................36
Section 9 Product Information .............................................................................37
9.1 Helios Gene Gun System ....................................................................................37
9.2 Spare Parts ...........................................................................................................38
9.3 Specifications ......................................................................................................38
Section 10 Appendices .............................................................................................39
10.1 Precipitation of RNA onto Microcarriers ...........................................................39
10.2 Replacing the O-rings and Inner Sleeve on the Helios Gene Gun.....................40
10.3 Replacing the O-ring on the Tubing Prep Unit...................................................41
10.4 Replacing Tubing Cutter Razor Blade and Unit Disassembly...........................42
10.5 Cleaning and Sterilizing the Helios Gene Gun...................................................43
10.6 Testing Cartridges for Microcarrier Penetration and Density ............................43
10.7 Quantitation of DNA in Cartridges.....................................................................45
10.8 References ...........................................................................................................46
10.9 Quick
Guide ........................................................................................................47