Bio-Rad Helios® Gene Gun System - Manual (Page 43)

Environmental
Operating 50 °F ( 10 °C) to 90 °F (32 °C) temp.
30–80 % humidity
Storage 32 °F (0 °C) to 140 °F (60 °C) temp.
10–90 % humidity
Tubing Prep Station Specifications
Physical
Dimensions Approximately 33.5” x 4”
Weight 11.2 lbs
Construction Aluminum and Acrylic
Electrical
Maximum Current 62 mA/125 mA peak
Voltage Input 220–240 Vac/100–120 Vac
Input Frequency 50/60 Hz
Functional
Input Pressure 30 psi N
2
Relief Pressure 30 psi ± 1.5 psi at regulator assembly
Regulator Adjustment TDB psi limit maximum
Speed 30 RPM nominal
Tubing Fill manual
Environmental
Operating 50 °F (10 °C) to 90 °F (32 °C) temp
30–80 % humidity
Storage 32 °F (0 °C) to 140 °F (60 °C) temp
10–90 % humidity
Section 10
Appendices
10.1 Precipitation of RNA onto microcarriers
Primary RNA transcripts and mRNA may be effectively delivered in vivo and in vitro
using the Helios Gene Gun System (Qiu et al., 1996). The procedure is similar to that for
DNA, but the precipitation step is performed with ammonium acetate and 2-propanol.
Materials
In addition to those identified in Section 5.2:
Purified RNA preparation (e.g. capped, polyadenylated, or oligo dT-selected mRNA).
5 M ammonium acetate
2-propanol
Experiments involving RNA require careful technique to prevent RNA degradation. In the stud-
ies published to date, it was not necessary to pre-treat the gold particles to prevent RNase contamination.
Procedure (sufficient for one length of Gold-Coat tubing):
1. In a 1.5 ml microfuge tube, weigh out 25 mg gold particles.
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