Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix - Manual (Page 6)

SsoAdvanced ™ Universal SYBR ® Green Supermix Instruction Manual iv | iv |
Educational Resources
Understanding the Basics
To learn more about similarities and differences between PCR and real-time PCR, understand
how SYBR
® Green and probe-based chemistries function, and see how data are collected and
interpreted, please view our interactive tutorial Understanding Real-Time PCR .
Reagent Evaluation and Comparison Tutorials
Reverse Transcription
When comparing two different reverse transcription kits, often not all characteristics of the
reverse transcription (RT) reaction are tested. The end result is that a decision is made using
a limited set of data and criteria. The following protocol and exercise have been written in an
effort to create a more robust, reliable, and reproducible method of testing sensitivity, efficiency,
and other critical characteristics when comparing reagent providers for reverse transcription
kits.
Reagent Comparison Guide for Real-Time PCR
To view an interactive tutorial and learn about reverse transcription chemistry, enzymes,
and priming methods, as well as how to perform a reagent comparison, please click here.
Understanding Reverse Transcription
Supermixes
When comparing two different supermixes, often not all characteristics of the PCR reaction
are tested. The end result is that a decision is made using a limited set of data and criteria.
The Reagent Comparison Guide for Real-Time PCR was written in an effort to create a more
robust, reliable, and reproducible method of testing sensitivity, efficiency, and other critical
assay characteristics when comparing reagent providers for use on real-time PCR systems.
To view an interactive tutorial and learn about supermix chemistry and enzymes, as well as how
to perform a reagent comparison, please click here.
Understanding Real-Time PCR Supermixes