Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix - Manual (Page 3)

SsoAdvanced ™ Universal SYBR ® Green Supermix Instruction Manual | i
Table of Contents
Sso7d Fusion Enzyme Technology iii
Educational Resources iv
Reagent Evaluation and Comparison Tutorials iv
Protocol
1
Sample Preparation Considerations 1
RNA Samples 1
RNA Integrity and Purity 1
DNA Samples 2
Plasmid Samples 2
Assay Design Considerations 3
Some Key Design Considerations 3
Procedure 4
Reaction Mix Preparation and Thermal Cycling Protocol 4
Real-Time PCR Validation for Gene Expression Experiments 5
Determining the Optimal Reference Gene 5
Determining the Dynamic Range of the Reverse Transcription Reaction 6
Determining the Real-Time PCR Performance Characteristics 9
Troubleshooting Guide
12
Ordering Information
21