Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix - Manual (Page 17)

| 11 SsoAdvanced ™ Universal SYBR ® Green Supermix Instruction Manual | 11
Dynamic Range
Determine the general trend of the slope where linearity (R 2 ) and efficiency are within acceptable
ranges, as specified above.
Sensitivity
Determine the lowest concentration of the serial dilution where replicate reproducibility is high
and the R
2 of the standard curve is ≥0.980.
Specificity
Evaluate the melt profiles when using SYBR ® Green–based assays. A single sharp peak with a
T
m
close to the calculated T
m
should be present.
Fig. 8. Example of a highly specific SYBR ® Green assay where the melt curve exhibits a single
melt peak around 85–87°C indicating a single PCR amplicon present.
1,200
60 70 80
–d
(R
FU
)/d
t
90
1,000
800
600
400
200
0