Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix - Manual (Page 18)

SsoAdvanced ™ Universal SYBR ® Green Supermix Instruction Manual 12 |
Troubleshooting Guide
Poor Nucleic Acid Yields
Review Tables 3 and 4 to determine if you are within an acceptable range of nucleic acid yield.
If your yields of RNA are considerably less than is typical for your sample type, reevaluate
your isolation method. For reference, typical yields from some mammalian tissues are listed in
tables 3 and 4.
Table 3. RNA yields.
Total RNA per Cell
Total DNA per Cell
5–30 pg Varies by genome
Table 4. RNA yields per mg of tissue.
Sample Type
Yield
Liver 4 µg
Spleen 4 µg
Heart 3 µg
Kidney 2 µg
Lung 2 µg
Brain 1.5 µg
Bone 50 ng
Adipose <10 ng
PCR Inhibitors/Oversaturation
If you suspect that your sample(s) contains PCR inhibitors, consider the following
corrective actions:
1. Evaluate your sample type to determine if any of the common inhibitors listed in the
following list may be present in your sample as carryover. If you suspect contamination,
re-purify the samples using a commercially available post-isolation cleanup kit.
2. Evaluate the A260/280 and A260/230 ratios. Refer to the RNA/DNA isolation section
(page 1) in this manual for further information.