C.B.S. Scientific DCX-700 - Manual (Page 19)

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Standard SDS Running Buffer, 20X for Reduced Samples (Cat. # FB60500)
* pH should be between 8.4 and 8.5 at 25° C.
Ingredient MW Molarity Qty/Liter
Tricine (free acid) 179.17 0.8M 143.4 g
Tris (free base) 121.14 1.2M 145.2 g
SDS (2%) 288.38 - 20.0 g
Sodium Meta-bisulfite 104.06 50mM 5.0 g
Ultrapure water (fill to) - - 1000ml
* For non-reduced samples (especially antibodies), omit the Sodium Meta-bisulfite
Standard SDS Running Buffer, 20X for Non-Reduced Samples, (Cat. #
FB50500)
* pH should be between 8.4 and 8.5 at 25° C.
Ingredient MW Molarity Qty/Liter
Tricine (free acid) 179.17 0.8M 143.4 g
Tris (free base) 121.14 1.2M 145.2 g
SDS (2%) 288.38 - 20.0 g
Ultrapure water (fill to) - - 1000ml
* For non-reduced samples (especially antibodies), omit the Sodium Meta-bisulfite
Turbo SDS Running Buffer, 20X, (Cat. # FB14500)
* pH should be between 8.3 and 8.4 at 25° C.
Ingredient MW Molarity Qty/Liter
MPS (free acid) 209.26 0.6M 125.6 g
Tris (free base) 121.14 1.2M 145.2 g
SDS (2%) 288.38 - 20.0 g
Sodium Meta-bisulfite 104.06 50mM 5.0 g
Ultrapure water (fill to) - - 1000 ml
* For non-reduced samples (especially antibodies), omit the Sodium Meta-bisulfite
Tris-Glycine-SDS Transfer Buffer, 10X, (Cat. # FB82500)
* pH should be between 8.4 and 8.6 at 25° C.
Ingredient MW Molarity Qty/Liter
Tris (free base) 121.14 0.25M 30.3 g
SDS (2%) 288.38 - 20.0 g
Glycine 75.07 1.92M 144.1 g
Ultrapure water (fill to) - - 1000 ml
6.4 Recommended ClearPAGE™ Buffer Formulations
As an alternative to the ClearPAGE buffers available for purchase, these formulations
may be used to prepare buffers yourself. Use high-quality, low-conductance ingredients.
Do NOT use acid or base to adjust the pH!