C.B.S. Scientific DCX-700 - Manual (Page 10)

10
Dual Cool DCX-700 Instructions 9-30-13
3.2 Running the Gel
1. Place core assembly into lower reservoir. The
anode (red) and cathode (black) electrodes
are color-coded on both the core/cassette
assembly and lower reservoir. See figure 7.
Ensure the red dot on the cassette assembly
is on the same side as the red receptacle on
the lower reservoir. Fill core upper reservoir
with freshly prepared buffer (~ 190mls).
If any buffer is spilled into banana jack
receptacles (outlined in yellow boxes in
figure 7) in lower reservoir, dry completely
using compressed air! Failure to do this
will result in accelerated banana jack cor-
rosion.
2. Important note if you are using the option-
al freezer cooling blocks: Use table below
to determine approximate buffer volume of
lower reservoir. Each freezer block displaces
125mls of buffer. Add buffer to lower cham-
ber only after freezer blocks are in place.
3. Pour only enough freshly prepared buffer
into lower chamber so that the final buffer
level (including freezer block displacement)
is just below bottom of sample wells. Using
a pipette or syringe, thoroughly flush out the
wells in the glass plate sandwich with buffer.
Load samples. If outer lanes do not contain
sample, it is recommended that you run
standards and/or fill outer lanes with loading
buffer to reduce smiling and wrap-around
effects.
matching
colors
7
Approximate buffer required for
lower reservoir
Number of freezer blocks in
lower reservoir
810 mls 0
685 mls 1
560 mls 2
stirring
bar