C.B.S. Scientific DCX-700 User Manual

Page 14

Dual Cool DCX-700 Instructions 9-30-13

SECTION 4
Alternate Protocol for Slab Gel Electrophoresis.

(Not using Pre-cast gels)

4.2

Preparing the Electrophoresis Unit when Using Gel Wrap™ Gasket

Casting Method

1. Place unit in authorized work area. Remove

safety cover from the assembled unit by simul-
taneously pressing down on white push pins
while lifting up on blue safety cover as shown in
figure 1.

Do not remove safety cover by pulling up on
leads!

2. Remove white core from lower reservoir by

grasping core with one hand and lifting directly
up as shown in figure 2.

3. Open doors on the core assembly by pulling up

on the white latches, as shown in figure 3.

4. Slide glass plate sandwich into the core assem-

bly with the notched plate facing in towards the
upper buffer reservoir as shown in figure 4.

5. If running one gel, slide white plastic adaptor

plate into the side without the gel.

6. Close doors and relatch by pressing down on

the white latches so that the assembly looks like
that shown in figure 5.

7. Place stirring bar into bottom of reservoir in stir-

ring corral (as shown in figure 7 on page 10).

8. If using freezer blocks take frozen blocks out of

freezer and insert into receptacles on either side
of lower reservoir.

9. For rest of protocol please

refer to instructions on
pages 10-11 (Sections 3.2
and 3.3) for running the
gel and removing it after
electrophoresis.