Eppendorf XplorerPlus Adjustment - Manual (Page 6)

General information on adjustment
Eppendorf Xplorer
® /Xplorer ® plus
English (EN)
6
Before the adjustment is changed, the existing adjustment must be checked in the factory settings using
distilled water.
The actual volume can be checked by weighing:
The density of distilled water is Approx. 0.9982 mg/μL at 20°C and 0.9965 mg/μL at 27°C.
If the set volume corresponds to the actual volume, no correction is necessary.
If there is a difference between the actual volume and the set volume of distilled water, please check the
following:
• Is there any liquid dripping from the tip?
• Is the pipette tip fitted leak-proof?
• Is the tip cone undamaged?
• Are the piston and the cylinder leak-proof?
• Does the temperature of the pipetted liquid correspond to:
– The temperature of the device?
– The ambient air temperature?
• Is the weighing location free from drafts?
• Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid?
• Has the correct numerical value for "Density liquids at weighing temperature" been used for the
calculation of the actual volume?
• Is the set volume correct?
• For very small volumes (<10 μL): is the fine balance sufficiently sensitive (balance resolution:
0.001 mg)?
• Were original epT.I.P.S pipette tips used for testing?
No adjustment changes are allowed unless you can answer all the questions with "yes".
In all other cases, the problems associated with the questions answered with "no" must be eliminated. If the
problem is remedied by exchanging a complete lower part or other parts that have an effect on the volume,
proper assembly must be verified by carrying out a gravimetric test. The systematic and random errors
which must be met are listed in the technical data.
Adequate leak tightness is ensured when no drop is formed at the pipette tip after aspiration
of the nominal volume with distilled water and a waiting time of approx. 15
s. Hold the pipette
vertically, without touching the pipette tip. Prewet the pipette tip several times.
The pipette tip which must be used as the test tip for checking technical data is listed in the
"Technical data" chapter in the operating manual.