Eppendorf XplorerPlus Adjustment - Manual (Page 12)

Change adjustment
Eppendorf Xplorer
® /Xplorer ® plus
English (EN)
12
2.7.1 1-point adjust.
After you enter the density, the selected volume and the corresponding mean value of the weighing results,
the Xplorer pipette will determine a correction factor. The factor is only correct for the selected volume and
the selected work technique. However, the factor is used for the entire volume range of the Xplorer pipette.
You must carry out a gravimetric test to check the validity of the factor.
Example of the 1-point adjust.
• The systematic error of a 40% salt solution should be improved by a 1-point adjustment for the
pipetting of 500 μL.
• You use a 1 000 μL Xplorer pipette with the volume range 50 – 1 000 μL with 1 000 μL epT.I.P.S. pipette
tips.
• The adjustment Factory settings is active for the Xplorer pipette.
• The density of the salt solution is, for example, 1.43 mg/μL (mg/μL = g/mL) at 20°C.
• In preliminary tests you have optimized your work technique (speed, handling blow-out (blow), angle of
inclination and working speed) so that you have achieved the best possible precision (random error) and
handling for your task.
• You have carried out at least 10 weighings for the 500 μL on a fine balance.
• The calculated mean value of the weighings is, for example, 709 mg.
Inputting the data
1. Move the selection dial to
Opt.
2. Mark the Adjustment option with the rocker. Press the Select softkey.
3. Mark the adjustment 1-point adjust. with the rocker. Press the Select softkey.
4. Set the density with the rocker (example: 1.43 mg/μL). Press the Next softkey.
5. Set the first volume with the rocker (example: 500 μL). Press the Next softkey.
6. Set the mean value of the weighings with the rocker (example: 709 mg). Press the Next softkey.
For the 5 mL and 10 mL Xplorer pipettes enter the values in mL and g.
7. Confirm the confirmation prompt with the Yes softkey.
8. To check this, turn the selection dial to a dispensing mode.
If the adjustment 1-point adjust. was successfully activated, the header of the display will show the symbols:
With a density of 1.43 mg/μL, 709 mg is equivalent to a volume of 495.8 μL. Without changing
the adjustment, pipetting 500 μL should result in a lesser finding of 4.2 μL (approx. 0.8 %).