Eppendorf XplorerPlus Adjustment - Manual (Page 11)

Change adjustment
Eppendorf Xplorer ® /Xplorer ® plus
English (EN)
11
2.6 Altitude
At increased altitudes there is a fall in air pressure so that the aspiration volume of a piston-stroke pipette is
reduced. The average air pressure at the corresponding height is used to correct the piston stroke of the
Xplorer pipette when this option is activated. The altitude can be selected in 250
m (820 ft) steps. The
maximum altitude that can be selected is 5 000 m. The Xplorer pipette is set to an altitude of 0 m on
delivery. This is the factory setting.
2.7 User adjustment
For your own adjustments you can select between a 1-point adjustment, 2-point adjustment and
3-point adjustment. For this you need to know the exact density of the liquid, and, depending on the
adjustment, the corresponding mean values of the weighing results for the selected 1 – 3 volumes. The
mathematical operations required for the correction are automatically carried out by the Xplorer pipette for
the 1
– 3-point adjustment. The changed adjustment is applied in all modes. Use a fine balance with a high
resolution to determine the weighing results. Dispensing operations under 10 μL require a balance with a
resolution of 0.001 mg.
The adjustments Ethanol 75% or Glycerol 50% , epTIPS long and Altitude can be combined
with each other. Before routine use, check whether the combination of adjustments meets
your requirements.
CAUTION! Falsified adjustment due to incorrect procedure.
The following steps require that the adjustment
Factory settings is activated for the Xplorer
pipette. This also applies when working with an epT.I.P.S.long or at an altitude other than 0 m
above sea level.
 Activate the adjustment Factory settings .
Refer to the general information before making the decision whether an adjustment should be
changed. This also contains information on verifying the determined gravimetric data.
In the case of very high densities (density above 2 mg/μL) or also if the vapor pressure is very
high, you should check as an alternative whether a more precise result could be achieved by
dispensing with the positive displacer Multipette/Repeater
(X)stream and the Combitips plus.