Eppendorf XplorerPlus Adjustment - Manual (Page 13)

Change adjustment
Eppendorf Xplorer ® /Xplorer ® plus
English (EN)
13
Abb. 2-1: Example: Correction of the piston stroke with "
Abb. 2-2: 1-point adjust.
Abb. 2-3: "
Fig. 2-3: Example: Correction of the piston stroke with " 1-point adjust. "
In this example, the piston stroke of the factory setting is increased by a factor. Strictly speaking, the
correction only applies to the test volume (example: 500
μL). However, the correction with this factor is
used for the entire volume range. The correction is different to that for a mechanical pipette. A correction
with a factor for a small volume results in a smaller stroke correction than with a large volume (see Fig. 2-1
on p. 13).
For comparison purposes: The stroke of a mechanical pipette can only be changed by a fixed volume (see
Fig. 2-4 on p. 14). This volume change applies to the entire measurement range of the mechanical pipette.
The existing adjustment is changed in parallel by a fixed amount. If, for example, the volume was increased
by 10
μL in order to reach the nominal volume of a 1 000 μL Research plus, this increase of 10 μL will also
apply to all volumes below. The correction of the piston stroke of the Xplorer pipette with a factor
corresponds rather to practical requirements.
What happens in the Xplorer pipette?
The Xplorer pipette uses the data entered to carry out the volume calculation and the correction of the
piston stroke.
Example values for the calculation of the
correction factor
• Mean value of the weighings: 709 mg
• Density of the liquid: 1.43 mg/μL
• Nominal volume: 500 μL
Calculation of the actual volume:
709
mg ÷ 1.43 mg/μL = 495.8 μL
Calculation of the volume difference:
500
μL – 495.8 μL = 4.2 µL
The piston stroke of the adjustment Factory settings is increased by a factor. The factor is formed from the
specified data.
min.
Factory setting
Legend:
1-point adjustment
max.
Piston stroke
of Xplorer
max.
Volume
display of Xplorer
25 % 50 % 75 %