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2 centrifuging in continuous operation, 3 short spin centrifugation, Centrifuging in continuous operation – Eppendorf Centrifuge 5427 R User Manual

Page 34: Short spin centrifugation, See short spin centrifugation on p. 34)

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Operation
Centrifuge 5427 R
English (EN)

34

5.6.2

Centrifuging in continuous operation

Perform the following steps in the sequence described.

1. Use time to set the continuous run.

The continuous run function can be set above 9:59

h or below 10

s. The timer shows

oo

to indicate

continuous operation.

2. Use temp to set the temperature.

3. With speed, set the g-force

(rcf) or speed

(rpm).

4. Press the start/stop key to start the centrifugation.

The symbol blinks in the display when the rotor is running.

5. Press the start/stop key to end the centrifugation.

During the braking process, centrifugation time flashes in the display.

When the rotor stops a signal tone is heard.

The centrifuge lid remains closed to maintain the sample temperature. You can open it by pressing
the open key.

5.6.3

Short spin centrifugation

Prerequisites

In the

SHORT

menu, the user sets whether the short spin centrifugation runs with the maximum

g-force

(rcf) or speed

(rpm) of the inserted rotor (

MAX

) or with a freely selected speed (

SET

).

The short spin centrifugation runs as long as the short key is pressed.

1. Only with short spin centrifugation with a set speed: set the g-force

(rcf)

or

speed

(rpm)

using

the

speed

arrow keys.

2. Use the temp arrow keys to adjust the temperature.

3. Start short spin centrifugation: hold the short key pressed down.

The symbol blinks in the display when the rotor is running.

All other keys are disabled during short spin centrifugation.

4. End short spin centrifugation: release the short key.

During the braking process, centrifugation time flashes in the display.

The centrifuge lid remains closed to maintain the sample temperature. Press the open key to open
the lid.

During the braking process, the short spin centrifugation can be restarted up to two times by
pressing the short key again.

The soft ramp is disabled during short spin centrifugation.