Eppendorf Centrifuge 5427 R - Manual (Page 33)

Operation
Centrifuge 5427 R
English (EN)
33
5.6 Centrifuging
Before using the Centrifuge 5427 R for the first time, familiarize yourself with the operating controls and
the display (see Overview of operating controls on p. 23).
Each of the centrifuging variants described here must be preceded by the preparation described above (see
Preparing for centrifugation on p. 27).
Please also note the instructions on cooling (see p. 30).
5.6.1 Centrifuging with preset time
Perform the following steps in the sequence described.
1. Use time to set the run time.
2. Use temp to set the temperature.
3. Use speed to set the g-force (rcf) or speed (rpm).
4. Press start/stop to start the centrifugation.
During centrifugation
• blinks in the display when the rotor is running.
• The current actual temperature will be displayed.
• The current g-force (rcf) resp. speed (rpm) of the rotor is displayed.
• The fast temp, open, short keys and the device menu are blocked during centrifugation.
• The total run time, temperature and speed (rpm) can be changed during the run. The display can be
switched between g-force (rcf) and speed (rpm).
• You can also terminate the centrifugation before the set run time has elapsed by pressing the start/
stop key.
End of centrifugation
• After completion of the set time, the centrifuge stops automatically. During braking the elapsed
centrifugation time is displayed flashing. When the rotor stops a signal tone is heard.
• The centrifuge lid remains closed to maintain the sample temperature. You can open it by pressing
the open key.
CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes!
 Before commencing centrifugation, follow the safety instructions relating to risks from
asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or
open tubes (see Warnings for intended use on p. 17).
WARNING! Risk of injury from improperly attached rotors and rotor lids.
 Only centrifuge with rotor and rotor lid firmly tightened.
 If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Immediately press the start/stop key to stop centrifuging.
During the run you can modify the total run time, the temperature and the g-force (rcf)/
speed (rpm).
The values on the display blink while changes are being made. The new parameters are
adopted immediately. If the time is changed during a run, the elapsed time will be subtracted
from this value. Please note that the shortest new total run time that can be set must be 2
min
longer than the elapsed time.
When using adapters, you can adjust the radius.