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Introduction – Techne TC-512 User Manual

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INTRODUCTION

The TC-512 is a gradient thermal cycler, designed to accurately control the temperatures of samples
between 4°C and 99°C. It has many scientific applications including DNA amplification, PCR

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, reverse

transcription and sequencing.

The TC-512 is programmed via a touch screen LCD graphics display. The user is able to input various
programs and parameters, including:

• Specified temperatures in °C
• Times for which the sample is to be held at the specified temperature
• Heating and cooling rates between the specified temperatures in °C/sec
• Gradient of temperatures across the thermal cycling block
• Incremental time or temperature increases or decreases when a cycle is repeated

Before using the TC-512 make sure you have read this manual carefully. If there is any doubt relating to
the proper use of this equipment, the staff at Bibby Scientific Ltd or your supplier will be happy to
assist you.

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Aspects of the PCR process are claimed in USA Patent Nos. 4,683,195; 4,683,202, and 4,965,188. Use of the TC-512 in such processes

does not convey a licence to practice the processes themselves.

TC512 Techne Manual 19/12/08 3:30 PM Page 4