Teledyne 6650 - Fluorescence Probe - Oil in Water analysis system User Manual

Page 41

UV-Photo-X Appendix

Teledyne

Analytical

Instruments

d) Wait 2 minutes for the output to stabilize and the unit

should display residual fluorescence level of the
probe/analyzer configuration.

7) Calculate the difference between the air and liquid fluorescence

levels. The difference is due to the change in refractive index
between air and the liquid sample.

A-1.3 Determination of the Process Fluorescence:

This experimental sequence is the first step in characterizing the

process fluorescence. The Fluorometer will use the Mode 2 initialization
used for the previous experimental measurements.

For laboratory analysis using a small vessel (recommend, P/N

53FCAL), the analyst will need to collect pull samples from the process
which covers the entire range of analyte concentrations. It is
recommended that at least six process samples be analyzed. The first
sample will be the process background material and should not contain
analyte of interest.

For pilot process testing, it is critical that the initial process liquid

be background material with no analyte of interest. The process can then
be run normally and monitored.

The steps outlined below are based on discrete sample analysis, but

can be easily modified for continuous process monitoring.

1) If the unit is not on, power-up the unit and allow warming up for

at least 1 hour. If the unit has been running, just begin the
sequence.

2) Place the background material in the sample vessel, wait 2

minutes and record the fluorescence level. This reading is your
background fluorescence and is a combination of the
fluorescence properties of the background material, stray light
and the probe/ analyzer fluorescence.

3) The dynamic range of the instrument, for the application

engineering sequence can be determined at this time using the
following equation:

Range Multiplier = 1999/Display Value

4) Gently wipe the probe tip with a lint free cotton swab or cloth

dampened with the process background material and gently rub