Teledyne 6650 - Fluorescence Probe - Oil in Water analysis system - Manual (Page 14)

Definition of Terms Model 6650
6 Teledyne Analytical Instruments
2.9 Signal-to-Background Ratio
Signal refers to the emission collected from a sample with known
analyte concentration by the fluorescence probe and monitored by the
analyzer. Background refers to the process liquid containing no analyte
of interest and any stray light present in the system. The signal-to-
background ratio should be calculated during the application engineering
phase of the project. Knowing this ratio will help determine when the
stray light level changes and/or the background material fluorescence
properties change. Refer to Appendix 1 for additional details.
2.10 Dynamic Range
Dynamic range refers to the range of concentrations an
instrument can read, from the minimum to the maximum detectable.
The minimum detectable concentration is determined by signal-to-noise
and signal-to-background ratios. The maximum detectable concentration
is determined by the compound’s chemistry and by factors such as
instrument sensitivity ranges, fluorescence (quantum) efficiency,
specificity of optical filters, etc.
2.11 Linearity
Fluorescence intensity is typically directly proportional (linear) to
concentration. There are, however, factors that affect this linear
relationship. For example, variations in temperature, pH, dissolved
oxygen content, stray light, turbidity, variation in the chemical
composition of the background, etc. can dramatically affect the linearity
of the fluorescence response. Practically, the linearity of the
measurement is determined during the application engineering phase of
the project. Refer to Appendix 1 for details.
2.12 Process Background
The liquid solution used to transport or sustain the analyte of
interest in the process. This solution has all the chemical constituents
found in the process except the analyte of interest.
2.13 Mode 1 Initialization
Mode 1 initialization is a method for setting up the Fluorometer for
process monitoring. Mode 1 initialization is used to setup the
Fluorometer when the fluorescence intensity versus analyte