YSI pHotoFlex Series - Manual (Page 53)

53 ba76126e01 03/2013
Note: Before using the test with your photometer for the first time, determine the reagent blank
value.
YSI model no.: PO4-4 TC
Category: KT (reaction cell test)
Cell: 16 mm
Measuring range: 0.06 - 3.50 mg/l PO
4
0.020 - 1.141 mg/l PO
4
-P
Display in mmol/l possible
Pipette 5 ml of sample
into a reaction cell and
close the cell with the
screw cap. Heat the cell in the
thermoreactor for 30
minutes at 120 °C. Remove the cell from the
thermoreactor and let it
cool down in a cell rack. With a pipette add 2.0 ml
Vario Sodium
hydroxide 1.00 N, close
the cell with the screw
cap and mix. Add the contents of a
Vario Phosphate RGT
F10 ml powder packet
and close the cell with
the screw cap.
Shake the cell for 10-
15 s. A small amount of
solid matter remains
undissolved. Allow to react for 2
minutes. Within 8 minutes after
the last reagent was
added:
Insert the cell in the
photometer cell shaft
and start measurement.
Notes:
• We recommend to determine a new reagent blank value (deionized water instead of sample) for each test package started.
• Clean all laboratory glassware with hydrochloric acid (approx. 20 %), then thoroughly rinse with deionized water. Do not
used any detergents that contain phosphate!
2:00
Phosphate, acid hydrolyzable
Program no.
336
*RN336*