YSI pHotoFlex Series - Manual (Page 50)

50 ba76126e01 03/2013
Note: Before using the test with your photometer for the first time, determine the reagent blank
value.
YSI model no.: Ntot2 TC (HR)
Category: KT (reaction cell test)
Cell: 16 mm
Measuring range: 10 - 150 mg/l N
Add the contents of a
VARIO TN Persulfate
Rgt. powder pack into a
TN Hydroxide HR
digestion cell. With a pipette add 0.5 ml
of sample, close the cell
with the screw cap and
mix vigorously for at least
30 s. A small amount of
solids may remain
undissolved. Heat the cell in the
thermoreactor at 120 °C
for 30 minutes. Remove the cell from the
thermoreactor and let it
cool down in a cell rack. Add the contents of a
VARIO TN Reagent A
powder pack, close the
cell with the screw cap
and mix for at least 15 s.
Allow to react for 3
minutes. Add the contents of a
VARIO TN Reagent B
powder pack, close the
cell with the screw cap
and mix for at least 15 s. Allow to react for 2
minutes. Pipette 2.0 ml of
prepared sample into a
TN Acid LR/HR
(Reagent C) reaction
cell and close the cell
with the screw cap. Mix the contents by
carefully swaying the cell
(10 x / for approx. 30 s
altogether).
Caution, the cell
becomes warm!
Note:
Always clean the powder funnel prior to adding a
reagent!
Allow to react for 5
minutes. Insert the cell in the
photometer cell shaft
and start measurement.
3:00 2:00
5:00
Nitrogen, total HR
Program no.
320
*RN320*