Phosphate, total, Po4-3 tc, 06 - 3.50 mg/l po – YSI pHotoFlex Series User Manual
Page 55: 16 mm, Rn316, 00 phosphate, total

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Note: Before using the test with your photometer for the first time, determine the reagent blank 
value.
YSI model no.:
PO4-3 TC
Category:
KT (reaction cell test)
Cell:
16 mm
Measuring range:
0.06 - 3.50 mg/l PO
4
0.020 - 1.141 mg/l PO
4
-P
Display in mmol/l possible
Pipette 5.0 ml of sample 
into a reaction cell.
Add the contents of a 
VARIO Potassium 
Persulfate F10 powder 
pack and close the cell 
with the screw cap.
Shake the cell vigorously 
to dissolve solids.
Heat the cell in the 
thermoreactor for 30 
minutes at 120 °C.
Remove the cell from the 
thermoreactor and let it 
cool down in a cell rack.
With a pipette add 2.0 ml 
of 1.54 N sodium 
hydroxide solution, close 
the cell with the screw 
cap and mix the contents 
by carefully swaying the 
cell.
Add the contents of a 
VARIO Phos 3 F10 
powder pack and close 
the cell with the screw 
cap.
To dissolve solids, shake 
the cell for approx. 10 to 
15 seconds. A small 
amount of solids may 
remain undissolved.
Allow to react for 2 
minutes.
Insert the cell in the 
photometer cell shaft 
and start measurement.
Notes:
•
We recommend to determine a new reagent blank value (deionized water instead of sample) for each test package started.
2:00
Phosphate, total
Program no.
316
*RN316*
