Nitrogen (total), Rn035, 00 nitrogen (total) – YSI pHotoFlex Series User Manual
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Note: Before using the test with your photometer for the first time, determine the reagent blank 
value.
YSI model no.:
14537
Category:
KT (reaction cell test)
Cell:
16 mm
Measuring range:
0.50 - 15.00 mg/l N
Pipette 10.0 ml of 
sample into an empty 
cell.
Add 1 level blue 
microspoon of N-1K.
Add 6 drops of N-2K, 
close cell with screw cap 
and mix.
Heat the cell in the 
thermoreactor for one 
hour at 120 °C.
Place the cell in the cell 
rack again and let it cool 
down to room 
temperature (=prepared 
sample).
Add 1 level blue 
microspoon of N-3K into 
a reaction cell and close 
the cell with the screw 
cap.
To dissolve solids, shake 
the cell vigorously for 1 
minute.
With a pipette add 1.5 ml 
of prepared sample very 
slowly, close with screw 
cap and mix vigorously.
Caution, cell becomes 
very hot!
Allow to react for 10 
minutes.
Insert the cell in the 
photometer cell shaft 
and start measurement.
Notes:
•
We recommend to determine a new reagent blank value (deionized water instead of sample) for each test package started.
•
Sample solution and reagents must have a temperature of 20-25 °C. Temper as necessary.
•
For further notes please refer to the package insert of the test.
10:00
Nitrogen (total)
Program no.
35
*RN035*
