Hoefer SE640 - Manual (Page 42)

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Gel recipes
The Laemmli gel recipes are for 30 ml of a single
concentration solution (enough for two 1.5 mm,
18 × 8 cm gels). Tabulated are ingredients and
volumes for relatively large pore gels (7.5 – 10% T
range) as well as smaller pore gels (12.5 – 15% T
range). A 4% stacking gel is common. The linear
gradient recipe is for 100 ml of solution. The
total volume needed depends on the number of
gels cast and the gel thickness; adjust as necessary.
All gels are crosslinked with 2.6% C.
Laemmli gel
(per 30 ml resolving gel solution, 5 ml stacking gel solution)
Separating gel Stacking gel
7.5% 10% 12.5% 15% 4%
Acrylamide stock (Solution #1) 7.5 ml 10.0 ml 12.5 ml 15.0 ml 0.67 ml
1.5 M TrisCl, pH 8.8 (Soln. #2) 7.5 ml 7.5 ml 7.5 ml 7.5 ml —
0.5 M TrisCl, pH 6.8 (Soln. #3) — — — — 1.25 ml
10% SDS (Solution #4) 0.3 ml 0.3 ml 0.3 ml 0.3 ml 0.05 ml
Deionized H
2
O 14.6 ml 12.1 ml 9.6 ml 7.1 ml 3.00 ml
10% APS (Solution #5) 150 µl 150 µl 150 µl 150 µl 25 µl
TEMED 10 µl 10 µl 10 µl 10 µl 2.5 µl
Final Volume 30.0 ml 30.0 ml 30.0 ml 30.0 ml 5.0 ml