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Operating instructions – Hoefer SE640 User Manual

Page 15

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Operating instructions

Gel casting and electrophoresis procedures
follow. Included are instructions for polyacryl-
amide gels (used with continuous or discontinu-
ous buffer systems), and gradient gels.

The gels required for the SE640 must be self-
cast. The Dual Gel Caster (included) holds two
gel sandwiches.

Prepare the gel sandwich

Glass plates, spacers, and clamp sets are sized
so that the assembled sandwich can be easily
aligned to create the seal required first to cast
the gel and then to run it. For best results, take
extra care to align all components when assem-
bling sandwiches
.

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