Hoefer SE640 - Manual (Page 26)

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4
Use a pipet to carefully fill each slot above the sample
wells with buffer in order to minimize disturbing the
samples. Then pour 100 ml of buffer into the chamber,
directing the buffer stream toward the side wall. Check
that no buffer is leaking around the gasket.
Lower buffer chamber
1
Place a magnetic spin bar into the lower buffer
chamber and place the unit on a magnetic stirrer.
Fill the lower chamber with a minimum of 2.1 liters
of buffer.
Optional: Prechill the buffer.
2
Fit the upper buffer chamber assembly into the lower
buffer chamber. Use a steady hand to avoid disturbing
the samples: Grasp the assembly in the casting stand
by the upper buffer chamber and carefully lower it
into the lower buffer chamber.
3
Inspect the installation and adjust the buffer levels.
Upper chamber. The electrode along the upper
chamber ridge must be submerged to a depth of
about 1 cm. This level requires 450 – 600 ml buffer:
just enough to cover the upper chamber ribs, but not
high enough to contact the banana plug.
Lower chamber. The lower buffer level chamber
requires a minimum of 2.1 liters and a maximum of
2.8 liters of buffer; enough to cover the wire on the
lower electrode fin, but maintaining a 1.5 cm clearance
from the underside of the upper buffer chamber.
4
Place the safety lid on the unit.