Hoefer SE640 - Manual (Page 37)

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Appendix A:
Laemmli system gels
The Laemmli system is the most common elec-
trophoresis protocol for SDS-denatured proteins.
The leading ion in this discontinuous buffer
system is chloride and the trailing ion is glycine.
Accordingly, the resolving gel and the stacking gel
contain Tris-Cl buffers (of different concentration
and pH), and the electrophoresis buffer contains
Tris-glycine. All buffers contain 0.1% SDS.
Polyacrylamide gel composition is indicated by two
different percentages:
% T = total acrylamide = g (acryl + bis) × 100
100 ml
% C = crosslinker = g (bis) × 100
g (acryl + bis)