Coomassie stain solutions coomassie stain solution, Destaining solution i, Destaining solution ii – Hoefer SE640 User Manual
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8. 0.025 M Tris, 0.192 M glycine, 0.1% SDS, pH 8.3
(Electrophoresis buffer, 5.0 liters)
Tris (FW 121.1)
0.025 M
15.1 g
Glycine (FW 75.07)
0.192 M
72.0 g
SDS (FW 288.4)
3.5 mM
5.0 g
Deionized H
2
O
to 5.0 liters
The pH of this buffer is approximately 8.3. Do not adjust pH.
Up to 20 liters can be prepared and stored for up to
2 months.
9. Coomassie stain solutions
Coomassie stain solution
(0.025% Coomassie Blue R-250,
40% Methanol, 7% Acetic acid, 2 liters)
Coomassie Blue R-250 (FW 826) 0.3 mM
0.5 g
Methanol (Stir until dissolved)
40% v/v
800.0 ml
Glacial acetic acid (99%)
7% v/v
140.0 ml
Deionized H
2
O
to 2.0 liters
Destaining solution I
(40% methanol, 7% acetic acid, 1 liter)
Methanol
40% v/v
400.0 ml
Glacial acetic acid (99%)
7% v/v
70.0 ml
Deionized H
2
O
to 1.0 liter
Destaining solution II
(7% acetic acid, 5% methanol)
Methanol
5% v/v
50.0 ml
Glacial acetic acid (99%)
7% v/v
70.0 ml
Deionized H
2
O
to 1.0 liter