Solutions, Acrylamide stock solution, 1.5 m triscl, ph 8.8 – Hoefer SE640 User Manual
Page 39: 0.5 m triscl, ph 6.8, 10% sds solution, 10% aps
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Solutions
1. Acrylamide stock solution
(30.8% T 2.6% C Bis, 200 ml)
Acrylamide (FW 71.08)
30% w/v
60 g
Bis* (FW 154.2)
0.8% w/v
1.6 g
Deionized H
2
O
to 200.0 ml
Store at 4 °C away from light.
*N,N’ Methylenebisacrylamide
2. 1.5 M TrisCl, pH 8.8
(4X Resolving gel buffer, 1 liter)
Tris (FW 121.1)
1.5 M
181.6 g
4 N HCl
to pH 8.8
Deionized H
2
O
to 1000 ml
3. 0.5 M TrisCl, pH 6.8
(4X Stacking gel buffer, 500 ml)
Tris (FW 121.1)
0.5 M
30.3 g
4 N HCl
to pH 6.8
Deionized H
2
O
to 500 ml
4. 10% SDS solution
(100 ml)
Sodium dodecylsulfate
0.35 M
10.0 g
(SDS) (FW 288.4)
Deionized H
2
O
to 100 ml
5. 10% APS
(Initiator, 1 ml)
Ammonium persulfate
0.44 mM
0.1 g
(APS) (FW 228.2)
Deionized H
2
O
to 1.0 ml
Fresh APS “crackles” when water is added. If yours does not,
replace it with fresh stock. Prepare just prior to use.
Note: Filter solutions 1– 4
through a 0.45 µm filter.
IMPORTANT! Refer to the mate-
rial safety data sheet (MSDS)
accompanying each chemical
for detailed handling and safety
information.
Caution! Acrylamide is a neuro-
toxin. Always wear gloves while
handling in any form and wear
a mask while weighing the
powder. Never mouth pipette
the solution.