Hoefer SE640 - Manual (Page 30)

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After electrophoresis
1
Once the tracking dye reaches the bottom of the
gel, turn off the power supply, disconnect the leads
and remove the safety lid. (Lift straight up to avoid
bending the banana plugs.)
2
Pull out the upper buffer chamber assembly. Pour the
buffer into a sink. Install the assembly in the dual
gel caster and then release the sandwiches by turning
and removing the cams.
3
Unscrew the clamps from the sandwiches and remove.
Gently loosen and then slide away both spacers. Use
the Hoefer Wonder Wedge plate separator tool to
separate the plates.
4
Carefully lift the glass plate with the gel attached.
Handle the gel with care to avoid damaging it. Invert
the plate and position the gel low over the staining
tray. Pry one corner of the gel away from the glass
and allow it to drop into the tray, or, if the gel is thick
enough to handle, lift it and place it into the tray. To
avoid splashing, add staining or fixative solution to the
tray after the gel is transferred.
5
Clean the unit as described in the next section.
Note: Use only flexible plastic
prying tools to avoid chipping
the glass plates.