Solutions, Acrylamide stock solution, 4x resolving gel buffer – Hoefer SE400 User Manual
Page 38: 4x stacking gel buffer, 10% sds solution, 10% aps (initiator)
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Solutions
1. Acrylamide stock solution
(30.8% T 2.6% C Bis, 200 ml)
Acrylamide (FW 71.08)
30% w/v
60.0 g
Bis* (FW 154.2)
0.8% w/v
1.6 g
Deionized H
2
O
to 200 ml
Store at 4 °C away from light.
*N,N’ Methylenebisacrylamide
2. 4X Resolving gel buffer
(1.5 M TrisCl, pH 8.8, 1 liter)
Tris base (FW 121.1)
1.5 M
181.5 g
HCl
to pH 8.8
Deionized H
2
O
to 1000 ml
Store up to 3 months at 4 °C in the dark.
3. 4X Stacking gel buffer
(0.5 M TrisCl, pH 6.8, 500 ml)
Tris base (FW 121.1)
0.5 M
30.3 g
HCl
to pH 6.8
Deionized H
2
O
to 500 m
Store up to 3 months at 4 °C in the dark.
4. 10% SDS solution
(100 ml)
SDS* (FW 288.4)
0.35 M
10.0 g
Deionized H
2
O
to 100 ml
Store up to 6 months at room temperature.
*Sodium dodecylsulfate
5. 10% APS (Initiator)
(1 ml)
APS* (FW 228.2)
0.44 mm
0.1 g
Deionized H
2
O
to 1.0 ml
Fresh APS “crackles” when water is added. If yours does not,
replace it with fresh stock. Prepare just prior to use.
*Ammonium persulfate
Note: Filter solutions 1–4
through a 0.45 µm filter.
Important! Refer to the
material safety data sheet
(MSDS) accompanying each
chemical for detailed handling
and safety information.