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Hoefer SE400 User Manual

Page 13

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p5

Spacer-Mate assembly template. Aligns spacers
for sandwich assembly.

Wonder Wedge Plate Separator Tool. Use to
disassemble gel sandwiches and to gauge spacer
and comb thickness.

Spacers. (May be ordered separately.) Spacers
determine the thickness of the gel. They are 2 cm
wide and are available in three thicknesses: 0.75,
1.0, and 1.5 mm.

Combs. (May be ordered separately.) Combs are
available in sizes that form 10, 12, 15, 20, or 28
wells. Preparative combs include 1 or 2 reference
wells in addition to a preparative well. Most
combs are available in all three thicknesses:
0.75, 1.0 and 1.5 mm.

All preparative combs, and combs with fewer
than 28 wells form wells that are 25 mm deep.
The 28-well comb forms wells that are only
15 mm deep so that wells do not collapse when
the comb is removed. The sample volume held
by each well depends on the gel thickness, well
depth and the number of wells per comb. Table
2 on page 15 lists volume per 1 mm depth for
wells created by each comb size. See the ordering
information for additional comb specifications.

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