Hoefer SE400 - Manual (Page 15)

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2. Operating instructions
Procedures for casting gels and electrophoretic
separation follow. Included are instructions for
both single percentage (homogeneous) and
gradient polyacrylamide gels. Appendix A lists
recipes and Appendix B gives a bibliography.
2.1 Gel casting preparation
2.1.1 Options—precast gels and self-cast gels
The SE400 unit accepts standard precast gels
purchased from commercial suppliers as well
as self-cast gels, which can be prepared using
the built-in casting stand. (To cast multiple
14 × 16 cm gels, the Multiple Gel Caster Kit,
which holds up to 10 sandwiches, and the Gel
Caster Kit, which holds up to four sandwiches,
can be ordered separately.) Gels for the SE410
must be self-cast.
Glass plates, spacers, and clamp sets are sized
so that the assembled sandwich can be easily
aligned to create the required seal. When assem-
bling sandwiches, take extra care to align all
components for best results.