Hoefer SE400 - Manual (Page 30)

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p22
Record each run
Keep a record of the current or voltage setting,
number and thickness of gels, buffer system, and
the starting and final current or voltage readings
for each run so that results can be compared.
Inconsistent results for the same system and
settings can indicate potential problems such as
current leaks, incorrect buffer concentrations,
high salt concentrations, or inconsistent
chemical quality.
Check band progress after 5 minutes, and
again after an hour, noting the migration rate
of the tracking dye. The run is complete when
the tracking dye reaches the bottom of the
gel. Watch the buffer level and, if necessary,
replenish it as required to keep the top electrode
submerged. (A small volume of buffer may
leak past a nicked plate or gasket, or buffer
may pass through the gel.)