Hoefer SE400 - Manual (Page 24)

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p16
Note: Before the first use,
disassemble the unit and wash
with a dilute solution of a
laboratory detergent and rinse
thoroughly, first with water and
then distilled water.
Note: To help hold the gasket
against the upper buffer
chamber, dab a small amount
of GelSeal at each end of the
gasket only and then install.
Important! A smooth fit between
the sandwich and gasket is
essential for a good seal.
2.4 Final assembly
1
Rinse both buffer chambers with water and distilled
water thoroughly before each use.
2
Install the gel sandwich in the lower buffer chamber
Release the sandwich from the caster by removing
both cams. Clean away any gel adhering to the
exterior of the gel sandwich. Install the sandwich
in the lower buffer chamber, clamp screws facing
toward the leads.
3
Carefully fill each sample well with electrophoresis
buffer, then underlay prepared sample into the
wells using a fine-tipped microsyringe or gel loading
pipet tip.
4
Attach the upper buffer chamber to the gel sandwich
Invert the upper chamber and press the slotted gasket
into the grooves for a precise fit.
Proceed with care so that the samples are not
disturbed: Lower the upper chamber onto the gel
sandwich. Install the cams, ridge pointing down, into
the cam holes as shown on page 17. Simultaneously
turn one cam clockwise and the other counterclock-
wise a full
180° to secure the assembly.