ChemoMetec NC-250 - Manual (Page 5)

991-0252 Rev. 1.1 February 2013 5
Quick Guide
1. Install the NucleoView™ NC-250™ software and the NucleoCounter® NC-250™ instrument as
described in the documentation on the USB stick containing the NucleoView™ NC-250™ software.
2. Start the NucleoView™ NC-250™ software by double-clicking NucleoView™ NC-250™ icon on the
desktop.
3. Wait while the NucleoCounter® NC-250™ instrument initializes. The slide tray can be heard aligning
during this process. When the LED on the instrument turns green, the instrument is ready.
4. Click the button (below the ‘F3’ button) and select the desired protocol.
5. Click the button to see the application note for the selected protocol.
Check that you have all the materials needed for this protocol (slides, reagents ...).
6. Mix your cells sample with the correct solution(s) and load an A2 or A8 sides.
7. Eject the slide tray and insert the slide.
8. Select the chambers on the slide to be analyzed by clicking in the checkboxes.
9. Optional: Write a descriptive text in the Sample ID field and enter a user name in the Operator
field.
10. Press the ‘Run’ button.
A few moments later, the results of the analysis will be displayed.
11. Repeat steps 6 to 8 to perform the same protocol again on a new sample or repeat from step 4 to
start a new type of analysis.