Volume calibration – ANKOM TDF Dietary User Manual
Page 86

Operator’s Manual
pg. 86
Rev E 12/2/14
When the fluid delivery is complete, the following screen will displayed.
9. Remove the beakers with fluid and weigh them.
10. Determine the amount of fluid delivered by doing the following. Subtract the weight of the empty beakers from the
weight of the beakers that contain fluid. Given that 1g of water equals 1 ml of water, convert the weight in grams to
volume in ml.
11. Press the number input box on the above screen (it shows “20.0” as the default) and enter the average ml of fluid
delivered; then press the
Enter button to capture the value and exit the input screen.
12. Press the
SET button. This modifies the Calibration Time and stores the new value in memory.
Temperature Sensing
The instrument uses temperature sensors on the heating paddles to monitor and control the temperature of the digestion phases.
Because the sensors have a tolerance range, you can set offsets to correct for differences in temperature sensors.
To calibrate the Temperature Sensing system, follow the steps below.
1. Start an IDF/SDF analysis on the instrument without using any samples. See the “IDF/SDF Analysis” section of this
manual for more details.
2. After 15 minutes of time has elapsed in the Protease phase of the IDF process, press the
Display Temperatures button
on the Status Screen.
3. With the front cover of the instrument in the down position (as it would be during normal operation), place a standard
Thermometer in each of the six IDF bags and record on a sheet of paper the temperature readings from the
Thermometer and from the sensors. The temperature should be 60°C at this time.
4. Abort the IDF/SDF run. The following screen will be displayed.
Volume Calibration
←
xx.x
Calibration Time
Enter average volume delivered
by pressing the number input,
entering the number, then pressing
the “SET” button.
SET
20.0
Volume Calibration
←
Final fluid delivery in progress...