ANKOM TDF Dietary - Manual (Page 56)

Operator’s Manual
pg. 56
Rev E 12/2/14
21. Adjust pH before the Protease digestion.
Just before the Protease digestion (after the bags have cooled to the appropriate temperature) the instrument will stop,
open Clamp Bar A, display the screen below, and make a sound to remind you to manually adjust pH.
When measuring/adjusting pH use a probe that can be easily rinsed (with Distilled
Water) to avoid loss of sample. If you add acid or base to adjust the pH, you must
mix the solution in order to get an accurate pH reading. To manually mix the
solution, press the outside of the bag with your fingers just above the clamp bar
multiple times.
After adjusting the pH, press the Continue button.
22. Manually measure/adjust pH after the Protease digestion.
One of the questions you are asked before starting the automated procedure is: “Check pH manually?” If you answered
“Yes” to this question, the instrument will stop after adding the required HCl, open Clamp Bar A, display the screen
below, and make a sound to remind you to manually measure pH and adjust if necessary.
When you have completed the pH measurement process, press the Continue button on the Status screen. You will see
“COMPLETE” next to “pH required?” in the upper left corner of the screen.
23. Make sure that each sample is completely rinsed down into the SDF filter bags (as needed).
Since the IDF Flow-thru bags are not weighed before and after the process, it is important to make sure that each sample
gets rinsed down into the SDF filter bag when the digestion process is complete. This is especially important for sticky
samples.
If you notice sample sticking to an IDF Flow-thru bag, use 78% EtOH in a wash bottle to rinse as needed.
Status
AOAC 985.29 TDF
Overall Progress Action in progress
IDF
Time remaining: Amylase/AMG Phase
XX:XX:XX Time remaining: XX:XX:XX
SDF
Time remaining:
XX:XX:XX
pH required?
Display
Temps/Pressures
YES
IDF
ABORT
PAUSE
SDF
ABORT
Check pH
then press continue
xx:xx:xx
Continue
AOAC 985.29 pH adjustment
Continue
Adjust pH to 7.5 +/- 0.2 by adding NaOH solution.
Press "Continue" when complete.