ANKOM TDF Dietary - Manual (Page 58)

Operator’s Manual
pg. 58
Rev E 12/2/14
28. Determine the Ash content within the TDF residue.
See the “Ash Determination” section of this manual for recommended procedures.
29. Calculate the % TDF value.
% TDF = [(R
1
+ R
2
)/2] - P - A - B
(M
1
+ M
2
)/2
= [(( f
F1
- f
S1
- D
1
) + ( f
F2
- f
S2
- D
2
))/2] - P
1
- (A
2
- D
2
) - B
(M
1
+ M
2
)/2
Where: M
1
, M
2
= Original wt of duplicate samples adjusted for pre-treatment fat and sugar losses (g)
R
1
, R
2
= Residue for duplicate samples (g)
f
F
= Final Filter Bag with residue (g)
f
S
= Initial Filter Bag (g)
D = Original wt of Diatomaceous Earth (g)
P = Protein of residue and bag (g)
A = Ash of residue and bag (g)
B = Blank (g)
= [(BR
1
+ BR
2
)/2] - P
B
- (A
B
- D
B
)
= [((f
BF1
- f
BS1
- D
B1
) + (f
BF2
- f
BS2
- D
B2
))/2] - P
B1
- (A
B2
- D
B2
)
BR
1
, BR
2
= Residue for duplicate blanks (g)
f
BF
= Final Blank Filter Bag (g)
f
BS
= Initial Blank Filter Bag (g)
P
B
= Protein of Blank Filter Bag (g)
A
B
= Ash of Blank Filter Bag (g)
D
B
= Original wt of Diatomaceous Earth in Blank Filter Bag (g)
X 100
X 100