Bio-Rad Aurum™ Total RNA 96 Kit - Manual (Page 8)

Table 1. Yield (per well) of total RNA from various samples using
the Aurum™ Total RNA 96 kit.
Starting Material Avg. Yield (µg)*
Cultured cells (1 x 10 6 )
3T3 7–10
HeLa 11–17
Bacterial (8 x 10 8 )
E. coli 5
B. cereus 5
Yeast (2 x 10 7 )
S. cerevisiae 9–11
Starting material amounts in parentheses are the maximum amounts recommended for use with the
Aurum™ Total RNA 96 kit.
*Yield figures are representative of a minimum of four full plate experiments.
Reagents Used With the Aurum™ Total RNA 96 Kit
• The low stringency wash solution is provided as a 5x concentrate. Add
4 volumes (240 ml) 95–100% ethanol to the low stringency wash
solution concentrate before initial use.
• Before using the RNA lysis solution, add 850 µl of b-mercaptoethanol to the
solution, for a final concentration of 1%.
• The RNase-free DNase I is provided as a lyophilized powder. Reconstitute
the DNase I by adding 250 µl 10 mM Tris, pH 7.5 (not supplied) to the vial.
Pipet up and down briefly to mix. Do not vortex. Store the reconstituted
DNase I at –20°C in a nonfrost-free freezer.
• Bacterial total RNA isolation with one RNA binding plate requires the use of
10 ml of TE (10 mM Tris, 1 mM EDTA, pH 7.5) for diluting the lysozyme. TE
and lysozyme are not supplied with the kit.
• Yeast total RNA isolation with one RNA binding plate requires the use of
100 ml of lyticase dilution buffer (1 M sorbitol, 0.1 M EDTA, pH 7.4,
0.1% b-mercaptoethanol), which is not supplied with the kit.
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