Bio-Rad Aurum™ Total RNA 96 Kit - Manual (Page 22)

18
Problem Possible Cause Recommended
Solution
Total RNA prep Incorrect use of wash Add the appropriate
performs poorly in solutions volume of 95–100%
downstream ethanol to the low
applications stringency wash
solution before initial
use
RNA is degraded See problem “RNA
degradation”
Ethanol contamination in Add 1–3 min to the
prep (eluate volumes purge time after the final
>60 µl) wash step
Blot RNA binding plate
drip directors with paper
towels; ensure that the
plate underside is dry
Inefficient drying Dry for a full 4 min and
ensure no droplets of
wash buffer remain on
the walls or the bottom
of the plate