Bio-Rad Aurum™ Total RNA 96 Kit - Manual (Page 12)

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2. Place the desired 96-well binding plate on the manifold top and apply the
recommended vacuum pressure for your application.
Manifold Elution Setup (Figure 3B)
3. When eluting from the purification plate, place the stage on the manifold
base, with the etched “This Side Up” facing up. Place a clean 96-well
microtiter collection plate securely on top of the stage and replace the
manifold top.
Note: If desired, waste can be collected into an optional pipet tip box lid
instead of draining directly into the vacuum trap.
4. After use, rinse the Aurum™ Vacuum Manifold with water and air dry or
wipe with paper towels. Failure to rinse the vacuum manifold at the end
of each use will cause the manifold to become cloudy and pitted.
Fig. 3. Manifold setup for plate processing.
A) 96-well wash; B) 96-well elution.
A
96-well
binding plate
Manifold top
Manifold top
96-well
collection plate
Stage
Manifold base Manifold base
B