Bio-Rad Aurum™ Total RNA 96 Kit - Manual (Page 5)

Section 1
Introduction
The Aurum™ Total RNA 96 kit rapidly purifies up to 192 total RNA samples
from biological samples (e.g. mammalian cells, yeast, or bacteria) with minimal
handling. Total RNA samples prepared using the Aurum™ Total RNA 96 kit
are suitable for use in a variety of downstream applications, including reverse
transcription-PCR (RT-PCR), quantitative real-time PCR, microarray analysis,
and northern blots. A DNase I digestion during the purification effectively
removes genomic DNA contamination from the preparation, eliminating the need
for a separate DNase digestion. All solutions and binding plates in the kit are
RNase-free, ensuring the integrity of the isolated total RNA. The Aurum™ Total
RNA 96 kit is designed specifically for use with the Aurum™ Vacuum Manifold.
Section 2
Kit Components
The Aurum™ Total RNA 96 kit contains the following components:
Components Quantity/Amount
Total RNA binding plates 2
Microtiter collection plates 2
Grow blocks 2
Sealing tape 2 pks of 4 pieces
DNase I (lyophilized) 2 vials
Lysis solution 85 ml
Low stringency wash solution 60 ml
(5x concentrate)
High stringency wash solution
150 ml
Elution solution 20 ml
DNase dilution solution 20 ml
1
Fig. 1. Selected components of the Aurum™ Total RNA 96 kit.
Total RNA binding plate
Microtiter
collection plate
Grow block