Bio-Rad Profinity IMAC Resins - Manual (Page 20)

Section 8
Preparing a Cartridge, and
Subsequent Purification
Prepare buffer sets for either the native or denaturing
purification protocols-using a single buffer set
throughout the procedure. To prepare the cartridge
for the procedure, remove the top closure and
connect the cartridge to the chromatography
system. Open the bottom closure and connect the
cartridge outlet to the system. Flush the packing
solution (20% EtOH) from the cartridge by running 2
column volumes (CV) of water at a flow rate of
2 ml/min (1 ml cartridge) or 10 ml/min (5 ml cartridge).
The cartridge is now ready for the purification steps.
Flow rates are given in ml/min and are specific to
the 1 ml cartridge. If a 5 ml cartridge is used for a
procedure, substitute the higher flow rate in the
method (refer to the table below).
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